A RP-HPLC method for simultaneous determination of calcium pantothenate and two preservatives methylparaben and propylparaben present in topical cream was developed. Different analytical columns with various stationary phases were tested. During method development, Supelco Discovery C18 column (125 mmx4.0 mm, 5 microm) and Zorbax SB-CN column (150 mmx4.6 mm, 5 microm) were tested. Both were not convenient for analytical separation because of the co-elution of calcium pantothenate with dead volume, and problems with the peak-shape of all components. Good separation was achieved using Zorbax TSM (250 mmx4.6 mm, 5 microm) and Hypersil ODS column (250 mmx4.6 mm, 5 microm), the latter was finally used for the analysis. The analysis time was 12 min, at flow rate 0.7 ml min-1. Chromatography was performed using binary mobile phase composed of methanol and phosphoric acid, pH 2.5, 65:35 (v/v). UV detection was accomplished at 214 nm. The method was validated according to ICH guideline recommendations. The method is suitable for practical routine analysis of commercially produced topical pharmaceutical preparations.
Dvorák J, Hájková R, Matysová L, Nováková L, Koupparis MA, Solich P. Dvorák J, et al. J Pharm Biomed Anal. 2004 Nov 15;36(3):625-9. doi: 10.1016/j.jpba.2004.07.018. J Pharm Biomed Anal. 2004. PMID: 15522540
Ali MS, Ghori M, Khatri AR. Ali MS, et al. J Pharm Biomed Anal. 2006 May 3;41(2):358-65. doi: 10.1016/j.jpba.2005.12.026. Epub 2006 Feb 7. J Pharm Biomed Anal. 2006. PMID: 16464558
Nováková L, Matysová L, Havlíková L, Solich P. Nováková L, et al. J Pharm Biomed Anal. 2005 Apr 29;37(5):899-905. doi: 10.1016/j.jpba.2004.09.012. J Pharm Biomed Anal. 2005. PMID: 15862664
Matysová L, Hájková R, Sícha J, Solich P. Matysová L, et al. Anal Bioanal Chem. 2003 Jun;376(4):440-3. doi: 10.1007/s00216-003-1756-x. Epub 2003 Feb 18. Anal Bioanal Chem. 2003. PMID: 12819846
Satínský D, Huclová J, Ferreira RL, Montenegro MC, Solich P. Satínský D, et al. J Pharm Biomed Anal. 2006 Feb 13;40(2):287-93. doi: 10.1016/j.jpba.2005.07.032. Epub 2005 Sep 13. J Pharm Biomed Anal. 2006. PMID: 16165338
Mahboubi A, Gholamreza Alviri M, Afshar M, Farhangi M. Mahboubi A, et al. Iran J Pharm Res. 2019 Spring;18(2):670-676. doi: 10.22037/ijpr.2019.1100681. Iran J Pharm Res. 2019. PMID: 31531050 Free PMC article.